A simple method for human peripheral blood monocyte isolation

Mem Inst Oswaldo Cruz. Mar-Apr 2000;95(2):221-3. doi: 10.1590/s0074-02762000000200014.


We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Separation / methods*
  • Centrifugation, Density Gradient / methods*
  • Humans
  • Monocytes*