An in vitro system from Plasmodium falciparum active in endogenous mRNA translation

Mem Inst Oswaldo Cruz. Mar-Apr 2000;95(2):231-5. doi: 10.1590/S0074-02762000000200017.

Abstract

An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell-Free System
  • Erythrocytes / parasitology
  • Plasmodium falciparum / chemistry*
  • Plasmodium falciparum / genetics
  • Plasmodium falciparum / metabolism
  • Protein Biosynthesis*
  • Protozoan Proteins / metabolism
  • RNA, Messenger / analysis*
  • RNA, Protozoan / analysis*

Substances

  • Protozoan Proteins
  • RNA, Messenger
  • RNA, Protozoan