Effects of Donor Age on Neoplastic Transformation of Adult Mouse Bladder Epithelium in Vitro

J Natl Cancer Inst. 1979 Apr;62(4):1017-23.


Neoplastic transformation of C57BL/lcrf-a' mouse bladder epithelium was induced in long-term primary cultures by a single 24-hour treatment with 7,12-dimethylbenz[a]anthracene on day 2 of culture. Transformed foci appeared earlier (40--60 days) and at a higher frequency (28%) in cultures from old donors (28--30 mo) compared with 100 days and 0.9% in cultures from young adult donors (5--7 mo). After transplantation into syngeneic mice, transformed cells produced carcinomas. Spontaneous epithelial transformation occurred in dimethyl sulfoxide-treated old cultures after the same interval (40--60 days) as in the carcinogen-treated cultures but at a lower frequency (5.3%). Spontaneous epithelial transformation did not occur in cultures from young donors.

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene
  • Age Factors
  • Aging*
  • Animals
  • Cell Transformation, Neoplastic*
  • Culture Techniques
  • Epithelium / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Transplantation
  • Neoplasms, Experimental / etiology
  • Transplantation, Isogeneic
  • Urinary Bladder Neoplasms / etiology*
  • Urinary Bladder Neoplasms / pathology


  • 9,10-Dimethyl-1,2-benzanthracene