Purification of rhodopsin kinase by recoverin affinity chromatography

Methods Enzymol. 2000;315:404-10. doi: 10.1016/s0076-6879(00)15857-4.
No abstract available

MeSH terms

  • Animals
  • Calcium-Binding Proteins* / isolation & purification
  • Cattle
  • Cell Line
  • Chromatography, Affinity / methods
  • Chromatography, Ion Exchange / methods
  • Cloning, Molecular / methods
  • Electrophoresis, Polyacrylamide Gel / methods
  • Escherichia coli
  • Eye Proteins*
  • G-Protein-Coupled Receptor Kinase 1
  • Hippocalcin
  • Lipoproteins*
  • Nerve Tissue Proteins*
  • Protein Kinases / isolation & purification*
  • Protein Kinases / metabolism
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Recoverin
  • Retina / enzymology
  • Rod Cell Outer Segment / chemistry
  • Rod Cell Outer Segment / enzymology*
  • Rod Cell Outer Segment / metabolism
  • Spodoptera
  • Transfection

Substances

  • Calcium-Binding Proteins
  • Eye Proteins
  • Lipoproteins
  • Nerve Tissue Proteins
  • Recombinant Proteins
  • Recoverin
  • Hippocalcin
  • Protein Kinases
  • G-Protein-Coupled Receptor Kinase 1