Tumour necrosis factor alpha increases melphalan concentration in tumour tissue after isolated limb perfusion

Br J Cancer. 2000 Mar;82(5):1000-3. doi: 10.1054/bjoc.1999.1032.


Several possible mechanisms for the synergistic anti-tumour effects between tumour necrosis factor alpha (TNF-alpha) and melphalan after isolated limb perfusion (ILP) have been presented. We found a significant sixfold increase in melphalan tumour tissue concentration after ILP when TNF-alpha was added to the perfusate, which provides a straightforward explanation for the observed synergism between melphalan and TNF-alpha in ILP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents, Alkylating / pharmacokinetics*
  • Disease Models, Animal
  • Drug Synergism
  • Extremities
  • Male
  • Melphalan / pharmacokinetics*
  • Neoplasm Transplantation
  • Neoplasms, Experimental / metabolism*
  • Perfusion
  • Rats
  • Rats, Inbred BN
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Antineoplastic Agents, Alkylating
  • Tumor Necrosis Factor-alpha
  • Melphalan