Molecular and virologic characteristics of lymphoid malignancies in children with AIDS

J Acquir Immune Defic Syndr. 2000 Feb 1;23(2):152-9. doi: 10.1097/00126334-200002010-00007.


Purpose: To characterize AIDS-associated lymphoid malignancies in children.

Patients and methods: We studied lymphomas and B-cell leukemias from 25 children with AIDS for immunoglobulin heavy chain gene clonality, c-myc oncogene abnormalities, and presence of HIV and Epstein-Barr virus.

Results: Monoclonal immunoglobulin gene rearrangements were identified in 22 of 23 cases tested, the single exception being one of mucosa-associated lymphoid tissue. Immunoglobulin gene/c-myc translocations were found in 3 of 4 cases of B (surface immunoglobulin-positive)-acute lymphoblastic leukemia, 8 of 11 small noncleaved cell lymphomas, and 1 of 5 large cell lymphomas. Mutations of c-myc were found in 2 of 13 small noncleaved cell lymphomas, 1 of 2 Epstein-Barr virus-positive mucosa-associated lymphoid tissue neoplasms, and 1 of 4 Epstein-Barr virus-negative B-acute lymphoblastic leukemia. Six small noncleaved cell lymphomas, both mucosa-associated lymphoid tissue neoplasms and one of large cell lymphoma had high levels of Epstein-Barr virus in tumor tissue. Hodgkin's disease tissue and B-acute lymphoblastic leukemia tumors were negative for EBV. Proviral HIV-1 was not detected in any tumor.

Conclusions: AIDS-associated lymphoid malignancies in children appear to have a different distribution of histologic subtypes than adult HIV-infected individuals, fewer large cell lymphomas occur in children. The small noncleaved cell lymphomas exhibit a lower frequency as well as different locations of c-myc mutations than AIDS-associated small noncleaved cell lymphomas in adults.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acquired Immunodeficiency Syndrome / complications*
  • Acquired Immunodeficiency Syndrome / genetics
  • Adult
  • Child
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Genes, Immunoglobulin
  • Genes, myc
  • HIV / isolation & purification
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • Leukemia, B-Cell / complications*
  • Lymphoma, AIDS-Related / complications*
  • Lymphoma, B-Cell, Marginal Zone / genetics
  • Male
  • Polymorphism, Single-Stranded Conformational