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. 1979 Jan 5;168(1):111-5.
doi: 10.1007/BF00267940.

High Frequency Transformation of Bacillus Subtilis Protoplasts by Plasmid DNA

High Frequency Transformation of Bacillus Subtilis Protoplasts by Plasmid DNA

S Chang et al. Mol Gen Genet. .

Abstract

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) transformants per microgram of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency.

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References

    1. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 - PubMed
    1. J Bacteriol. 1975 Jun;122(3):987-93 - PubMed
    1. Biochemistry. 1973 Jul 31;12(16):3055-63 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1974;14(0):39-100 - PubMed

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