The cancer germ-line genes MAGE-1, MAGE-3 and PRAME are commonly expressed by human myeloma cells

Eur J Immunol. 2000 Mar;30(3):803-9. doi: 10.1002/1521-4141(200003)30:3<803::AID-IMMU803>3.0.CO;2-P.


In this study, we have investigated the mRNA expression of the cancer germ-line genes MAGE, BAGE, GAGE, RAGE and the tumor-overexpressed gene PRAME by human myeloma cell lines and malignant plasma cells from patients with multiple myeloma (MM). By reverse transcription-PCR, we show that all myeloma cell lines (n = 16) express at least one of these genes, except RAGE-1 that was never expressed. We show that malignant plasma cells from the majority of MM patients (n = 21) expressed MAGE-1, MAGE-3 and PRAME. On the contrary, polyclonal reactive plasma cells did not express any of these genes. By flow cytometry, we show that mage-1 protein is expressed within myeloma cells and cell lines and that anti-mage-1.HLA-A1 cytotoxic T lymphocytes efficiently killed MAGE-1+HLA-A1+ MDN myeloma cells. Taken together, our data show that mage-1 and mage-3 could constitute specific targets for tumor immunotherapy of MM patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm
  • B-Lymphocytes / immunology
  • Eye Proteins / genetics
  • Flow Cytometry
  • Gene Expression
  • Humans
  • MART-1 Antigen
  • Melanoma-Specific Antigens
  • Multiple Myeloma / genetics*
  • Multiple Myeloma / immunology*
  • Multiple Myeloma / metabolism
  • Neoplasm Proteins / genetics*
  • Oncogenes*
  • Plasma Cells / immunology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • T-Lymphocytes, Cytotoxic / immunology
  • Tumor Cells, Cultured


  • Antigens, Neoplasm
  • BAGE protein, human
  • Eye Proteins
  • GAGE1 protein, human
  • GAGE2A protein, human
  • MAGEA1 protein, human
  • MAGEA3 protein, human
  • MART-1 Antigen
  • MLANA protein, human
  • Melanoma-Specific Antigens
  • Neoplasm Proteins
  • PRAME protein, human
  • RNA, Messenger
  • RNA, Neoplasm