Cloning and expression throughout mouse development of mfat1, a homologue of the Drosophila tumour suppressor gene fat

Dev Dyn. 2000 Mar;217(3):233-40. doi: 10.1002/(SICI)1097-0177(200003)217:3<233::AID-DVDY1>3.0.CO;2-O.


We present the entire sequence of the mouse Fat orthologue (mFat1), a protein of 4,588 amino acids with 34 cadherin repeats, 27 potential N-glycosylation sites, five EGF repeats and a laminin A G-motif in its extracellular domain. A single transmembrane region is followed by a cytoplasmic domain containing putative catenin-binding sequences. mFat1 shows high homology to human FAT and lesser homology to Drosophila Fat. The sequence of this giant cadherin suggests that it is unlikely to have a homophilic adhesive function, but may mediate heterophilic adhesion or play a signalling role. Expression analysis shows that the mfat1 gene is expressed early in pre-implantation mouse development, at the compact eight cell stage. Whole-mount and section in situ analyses show that transcripts are widely expressed throughout post-implantation development, most notably in the limb buds, branchial arches, forming somites, and in particular in the proliferating ventricular zones in the brain, being down-regulated as cells cease dividing. RT-PCR detects widespread expression in the adult suggesting a role in proliferation and differentiation of many tissues and cell types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / embryology
  • Brain / metabolism
  • Cadherins / genetics*
  • Cloning, Molecular
  • DNA, Complementary
  • Drosophila
  • Embryonic Development
  • Embryonic and Fetal Development
  • Female
  • Gene Expression
  • Genes, Tumor Suppressor*
  • Humans
  • Membrane Proteins / genetics*
  • Mice
  • Molecular Sequence Data
  • Pregnancy
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Zygote


  • Cadherins
  • DNA, Complementary
  • FAT1 protein, human
  • Membrane Proteins
  • fat1 protein, mouse

Associated data

  • GENBANK/AJ250768