Abstract
Binding of Trypanosoma cruzi trypomastigotes to laminin is enhanced by galectin-3, a beta-galactoside binding lectin. The galectin-3 enhanced binding of trypanosomes to laminin is inhibited by lactose. Co-immunoprecipitations indicate that galectin-3 binds to the 45, 32 and 30 kDa trypanosome surface proteins. Binding of galectin-3 to the 45, 32 and 30 kDa surface proteins is inhibited by lactose. Polyclonal and a monoclonal antibodies to galectin-3 immunoprecipitated a major 64 kDa trypanosome surface protein. T. cruzi monoclonal antibody to mucin recognized the 45 kDa surface protein. The 45, 32 and 30 kDa surface proteins interact with galectin-3 in order to enhance trypanosome adhesion to laminin.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antigens, Differentiation / immunology
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Antigens, Differentiation / metabolism*
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Antigens, Differentiation / pharmacology
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Blotting, Western
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Cell Adhesion / drug effects
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Collagen / metabolism
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Cross Reactions / immunology
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Extracellular Matrix / drug effects
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Extracellular Matrix / metabolism*
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Extracellular Matrix / parasitology
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Galectin 3
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Humans
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Lactose / metabolism
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Lactose / pharmacology
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Laminin / metabolism*
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Molecular Weight
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Mucins / chemistry
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Mucins / immunology
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Mucins / metabolism
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Precipitin Tests
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Protein Binding / drug effects
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Trypanosoma cruzi / chemistry
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Trypanosoma cruzi / drug effects
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Trypanosoma cruzi / metabolism*
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Trypanosoma cruzi / physiology
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Variant Surface Glycoproteins, Trypanosoma / chemistry
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Variant Surface Glycoproteins, Trypanosoma / immunology
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Variant Surface Glycoproteins, Trypanosoma / metabolism*
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation
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Galectin 3
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Laminin
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Mucins
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Variant Surface Glycoproteins, Trypanosoma
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Collagen
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Lactose