In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats

FEBS Lett. 2000 Mar 31;470(3):325-30. doi: 10.1016/s0014-5793(00)01337-5.


To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt-Farber model in rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Acetylaminofluorene / toxicity*
  • Animals
  • Cell Division / drug effects
  • Disease Models, Animal*
  • Gene Transfer Techniques
  • Hepatectomy*
  • Hepatocyte Growth Factor / genetics
  • Hepatocyte Growth Factor / physiology*
  • Immunohistochemistry
  • Liver / cytology*
  • Liver / drug effects*
  • Liver / metabolism
  • Liver / surgery
  • Male
  • Proliferating Cell Nuclear Antigen / analysis
  • Proto-Oncogene Proteins c-kit / genetics
  • Proto-Oncogene Proteins c-met / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Inbred F344
  • Stem Cell Factor / genetics
  • Time Factors


  • Proliferating Cell Nuclear Antigen
  • RNA, Messenger
  • Stem Cell Factor
  • Hepatocyte Growth Factor
  • 2-Acetylaminofluorene
  • Proto-Oncogene Proteins c-kit
  • Proto-Oncogene Proteins c-met