Survival and growth of Burkholderia cepacia within the free-living amoeba Acanthamoeba polyphaga

Eur J Clin Microbiol Infect Dis. 2000 Feb;19(2):121-3. doi: 10.1007/s100960050442.


The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia.

MeSH terms

  • Acanthamoeba / growth & development
  • Acanthamoeba / microbiology*
  • Animals
  • Burkholderia cepacia / growth & development*
  • Colony Count, Microbial
  • Culture Media
  • Fluorescent Antibody Technique
  • Humans


  • Culture Media