Abstract
rRNA-based molecular phylogenetic techniques were used to identify the bacterial species present in the ear fluid from a female patient with otitis externa. We report the identification of Staphylococcus intermedius from the patient and a possible route of transmission. Analysis of 16S ribosomal DNA restriction fragment length polymorphisms indicated that the dominant species present was S. intermedius. A pet dog owned by the patient also was tested and found to harbor S. intermedius. In humans, the disease is rare and considered a zoonosis. Previously, S. intermedius has been associated with dog bite wounds, catheter-related injuries, and surgery. This study represents the first reported case of a noninvasive infection with S. intermedius.
Publication types
-
Case Reports
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adult
-
Animals
-
DNA, Bacterial / analysis
-
DNA, Bacterial / genetics
-
DNA, Ribosomal / analysis
-
DNA, Ribosomal / genetics
-
Dog Diseases / microbiology
-
Dog Diseases / transmission*
-
Dogs
-
Female
-
Humans
-
Molecular Sequence Data
-
Otitis Externa / microbiology*
-
Phylogeny*
-
Polymerase Chain Reaction
-
Polymorphism, Restriction Fragment Length
-
RNA, Ribosomal, 16S / genetics
-
Sequence Analysis, DNA
-
Staphylococcal Infections / microbiology
-
Staphylococcal Infections / transmission*
-
Staphylococcal Infections / veterinary
-
Staphylococcus / classification*
-
Staphylococcus / genetics
-
Staphylococcus / isolation & purification
-
Zoonoses*
Substances
-
DNA, Bacterial
-
DNA, Ribosomal
-
RNA, Ribosomal, 16S
Associated data
-
GENBANK/AF193881
-
GENBANK/AF193882
-
GENBANK/AF193883
-
GENBANK/AF193884
-
GENBANK/AF193885
-
GENBANK/AF193886
-
GENBANK/AF193887
-
GENBANK/AF193888