Induction of human cytotoxic T lymphocytes by artificial antigen-presenting cells

Nat Biotechnol. 2000 Apr;18(4):405-9. doi: 10.1038/74455.


The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were retrovirally transduced with a single HLA-peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any specific peptide.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Adoptive Transfer
  • Animals
  • Antigen-Presenting Cells / immunology*
  • CD58 Antigens / genetics
  • CD8-Positive T-Lymphocytes / immunology
  • Dendritic Cells / immunology
  • Genetic Vectors
  • HLA-A2 Antigen / genetics*
  • HLA-A2 Antigen / immunology
  • HLA-B7 Antigen / genetics
  • Humans
  • Intercellular Adhesion Molecule-1 / genetics
  • Lymphocyte Activation
  • Mice
  • Recombinant Proteins / immunology
  • Retroviridae
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / pathology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Transfection


  • CD58 Antigens
  • HLA-A2 Antigen
  • HLA-B7 Antigen
  • Recombinant Proteins
  • Intercellular Adhesion Molecule-1