Abstract
Meltrin beta (ADAM19) is a member of the metalloprotease-disintegrin family. We report here chromosomal mapping of the mouse and rat meltrin beta genes and cloning and analysis of the mouse upstream regulatory regions. The meltrin beta transcript shows a spatially and temporally restricted expression pattern during morphogenesis, indicating that the actions of this membrane-bound protease are regulated, at least in part, at the transcriptional level. Analysis of the promoter revealed positive and negative regulatory regions upstream of the gene. The former includes a GC-box that appears to be a critical cis-element for activation of the promoter in muscle cells.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADAM Proteins
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Chromosome Mapping
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Cloning, Molecular
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DNA, Complementary
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Disintegrins*
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Green Fluorescent Proteins
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In Situ Hybridization, Fluorescence
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Luminescent Proteins / genetics
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Membrane Proteins / genetics*
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Metalloendopeptidases*
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Metalloproteases*
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Mice
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Molecular Sequence Data
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Muscle Proteins / genetics*
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Promoter Regions, Genetic
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Rats
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Regulatory Sequences, Nucleic Acid*
Substances
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DNA, Complementary
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Disintegrins
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Luminescent Proteins
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Membrane Proteins
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Muscle Proteins
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Green Fluorescent Proteins
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Metalloproteases
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ADAM Proteins
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ADAM19 protein, human
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Adam19 protein, mouse
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Metalloendopeptidases