Intracerebral injection of autologous whole blood in rats: time course of inflammation and cell death

Neurosci Lett. 2000 Apr 14;283(3):230-2. doi: 10.1016/s0304-3940(00)00971-x.

Abstract

Intracerebral hemorrhage is associated with stroke and head trauma. The purpose of this study was to study brain inflammation and cell death in adult rats 1 h to 4 weeks after injection of blood into the striatum. Terminal dUTP nick-end-labeling positive dying cells were evident 4 h to 4 weeks post-hemorrhage. Neutrophil infiltration was brief and peaked at 48 h. CD8a immunoreactive lymphocytes, possibly natural killer cells, became apparent at 48 h and persisted for 1 week. Microglial reaction was evident at 4 h and persisted for 4 weeks. We conclude that extravascular blood causes a mixed inflammatory cell reaction in brains that is maximal from 48-72 h following hemorrhage. This is associated with death of brain cells over a prolonged period of at least 4 weeks.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Transfusion, Autologous* / methods
  • Cell Death
  • Cell Movement
  • Cerebral Hemorrhage / blood*
  • Cerebral Hemorrhage / pathology*
  • Corpus Striatum / pathology
  • In Situ Nick-End Labeling
  • Inflammation / blood
  • Inflammation / pathology
  • Injections, Intraventricular
  • Leukocyte Count
  • Lymphocytes / pathology
  • Male
  • Microglia / pathology
  • Neutrophils / pathology
  • Rats
  • Rats, Sprague-Dawley