Abstract
Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alphaproteobacteria / genetics
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Antigens, Bacterial / biosynthesis
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Antigens, Bacterial / isolation & purification
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Bacterial Proteins*
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Fucosyltransferases / genetics
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Fucosyltransferases / metabolism*
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Lipopolysaccharides / biosynthesis
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Lipopolysaccharides / isolation & purification
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plants / microbiology*
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Rhizobium leguminosarum / genetics
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Rhizobium leguminosarum / metabolism*
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Symbiosis / genetics*
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Symbiosis / physiology
Substances
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Antigens, Bacterial
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Bacterial Proteins
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Lipopolysaccharides
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Plant Proteins
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lipid-linked oligosaccharides
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noIL protein, Rhizobium etli
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Fucosyltransferases
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NodZ protein, bacteria