A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NoIL in Rhizobium leguminosarum

Mol Plant Microbe Interact. 2000 Apr;13(4):475-9. doi: 10.1094/MPMI.2000.13.4.475.


Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaproteobacteria / genetics
  • Antigens, Bacterial / biosynthesis
  • Antigens, Bacterial / isolation & purification
  • Bacterial Proteins*
  • Fucosyltransferases / genetics
  • Fucosyltransferases / metabolism*
  • Lipopolysaccharides / biosynthesis
  • Lipopolysaccharides / isolation & purification
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants / microbiology*
  • Rhizobium leguminosarum / genetics
  • Rhizobium leguminosarum / metabolism*
  • Symbiosis / genetics*
  • Symbiosis / physiology


  • Antigens, Bacterial
  • Bacterial Proteins
  • Lipopolysaccharides
  • Plant Proteins
  • lipid-linked oligosaccharides
  • noIL protein, Rhizobium etli
  • Fucosyltransferases
  • NodZ protein, bacteria