Premature termination codons destabilize ferredoxin-1 mRNA when ferredoxin-1 is translated

Plant J. 2000 Mar;21(6):563-9. doi: 10.1046/j.1365-313x.2000.00705.x.

Abstract

Ferredoxin-1 (Fed-1) mRNA is poorly translated in dark-treated tobacco (Nicotiana tabacum) leaves, resulting in destabilization of Fed-1 mRNA and a differential light/dark accumulation of the mRNA. Insertion of nonsense codons within the Fed-1 coding sequence disrupts the light regulation of Fed-1 mRNA abundance. Here we show that the nonsense codon effect results primarily from lowering the Fed-1 mRNA stability in light-treated leaf tissue and in rapidly growing tobacco cell cultures, but not in dark-treated leaf tissue. These results suggest that nonsense codons trigger a decay pathway distinct from that seen for Fed-1 mRNA in the dark. We propose that nonsense-mediated decay of nonsense-containing Fed-1 mRNA occurs in light-treated leaves and in non-photosynthetic tobacco culture cells where Fed-1 mRNA is being actively translated.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cells, Cultured
  • Codon, Terminator*
  • Ferredoxins / genetics*
  • Mutation
  • Peas / genetics*
  • Peas / metabolism
  • Protein Biosynthesis*
  • RNA, Messenger / metabolism*

Substances

  • Codon, Terminator
  • Ferredoxins
  • RNA, Messenger