Description of strain 3CB-1, a genomovar of Thauera aromatica, capable of degrading 3-chlorobenzoate coupled to nitrate reduction

Int J Syst Evol Microbiol. 2000 Mar:50 Pt 2:551-558. doi: 10.1099/00207713-50-2-551.

Abstract

A Gram-negative bacterium, strain 3CB-1, isolated from a 3-chlorobenzoate enrichment culture inoculated with a sediment sample is capable of degrading various aromatic compounds and halogenated derivatives with nitrate as electron acceptor. Compounds capable of serving as carbon and energy sources include 3-chlorobenzoate, 3-bromobenzoate, 2-fluorobenzoate, 4-fluorobenzoate, benzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 3-aminobenzoate, protocatechuate, m-cresol and p-cresol. Oxygen, nitrate and nitrite were used as electron acceptors for growth. Cells are Gram-negative short rods with peritrichous flagellation. The predominant fatty acids are cis-9-hexadecenoic acid (16:1 omega 7c), hexadecanoic acid (16:0), octadecanoic acid (18:0), octadecenoic acid (18:1), 3-hydroxydecanoic acid (10:0 3OH) and dodecanoic acid (12:0). The sequence of the 16S rRNA gene, as well as the fatty acid composition, indicate that the strain is a member of the genus Thauera in the beta-subclass of the Proteobacteria and very close to Thauera aromatica. DNA-DNA hybridization and nutrient screening indicate that strain 3CB-1 is a genomovar of Thauera aromatica with the proposed name Thauera aromatica genomovar chlorobenzoica.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biodegradation, Environmental
  • Chlorobenzoates / metabolism*
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Genes, rRNA
  • Molecular Sequence Data
  • Nitrates / metabolism*
  • Nucleic Acid Hybridization
  • Oxidation-Reduction
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Thauera / classification*
  • Thauera / genetics
  • Thauera / isolation & purification
  • Thauera / metabolism
  • Water Microbiology

Substances

  • Chlorobenzoates
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Nitrates
  • RNA, Ribosomal, 16S
  • 3-chlorobenzoic acid

Associated data

  • GENBANK/AF123264