Interferon-gamma induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases

Parasitology. 2000 Mar:120 ( Pt 3):281-7. doi: 10.1017/s0031182099005521.

Abstract

Bidirectional signalling between Trypanosoma brucei brucei and CD8+ T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-gamma. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-gamma in a dose-response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-gamma-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Blotting, Western
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Inhibitors / chemistry
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Interferon-gamma / pharmacology*
  • Interferon-gamma / physiology
  • Leukocytes, Mononuclear / immunology
  • Protein-Tyrosine Kinases / physiology*
  • Protozoan Proteins / metabolism*
  • Rats
  • Rats, Inbred Lew
  • Rats, Sprague-Dawley
  • Signal Transduction
  • Trypanosoma brucei brucei / immunology
  • Trypanosoma brucei brucei / physiology*
  • Trypanosomiasis, African / parasitology*
  • Tyrphostins / chemistry

Substances

  • Antibodies, Monoclonal
  • Enzyme Inhibitors
  • Protozoan Proteins
  • T lymphocyte triggering factor, Trypanosoma brucei
  • Tyrphostins
  • tyrphostin 47
  • Interferon-gamma
  • Protein-Tyrosine Kinases