A pathophysiological study of abdominal organs following intraperitoneal injections of chloral hydrate in rats: comparison between two anaesthesia protocols

Lab Anim. 2000 Jan;34(1):84-90. doi: 10.1258/002367700780578082.


Chloral hydrate (CH) is used as an anaesthetic agent in laboratory rats. Side effects occurring with high concentrations have mainly occurred in abdominal organs. The objective of the present study was to minimize these side effects following intraperitoneal administration of CH using lower concentrations. Animals were evaluated using different procedures including a general necropsy, intraperitoneal white cell counts, histology and duodenal peristalsis and acetylcholine-induced contractions. Results clearly show that lower concentrations of CH while keeping the same anaesthetic dose (400 mg/kg) will minimize the irritancy of CH on abdominal organs while providing the same level of anaesthesia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdominal Muscles / drug effects
  • Abdominal Muscles / pathology
  • Anesthetics, Intravenous / administration & dosage
  • Anesthetics, Intravenous / pharmacology*
  • Animals
  • Chloral Hydrate / administration & dosage
  • Chloral Hydrate / pharmacology*
  • Female
  • Injections, Intraperitoneal / veterinary
  • Intestine, Small / drug effects
  • Intestine, Small / physiology
  • Male
  • Muscle Contraction / drug effects
  • Muscle, Smooth / drug effects
  • Peristalsis / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Viscera / anatomy & histology
  • Viscera / drug effects*
  • Viscera / physiology


  • Anesthetics, Intravenous
  • Chloral Hydrate