Abstract
We have developed a specific and sensitive method aiming at docetaxel (Taxotere) determination in plasma of treated patients. This involved solid-phase extraction of 1 ml of plasma onto carboxylic acid (CBA) grafted silica cartridges followed by reversed-phase liquid chromatography with UV detection. The best selectivity was obtained through the use of C18 Uptisphere as stationary phase. The low limit of quantitation obtained (LOQ: 5 ng/ml) allowed measurements of docetaxel up to 24 hours after one-hour infusions with low dosages of drug (60 mg/m2). The method was applied successfully to monitor docetaxel plasma levels within two protocols associating fixed dosages of either methotrexate or gemcitabine with escalating doses of Taxotere.
Publication types
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Clinical Trial
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Clinical Trial, Phase I
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Research Support, Non-U.S. Gov't
MeSH terms
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Antimetabolites, Antineoplastic / administration & dosage
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Antimetabolites, Antineoplastic / blood
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Antineoplastic Agents, Phytogenic / blood*
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Antineoplastic Combined Chemotherapy Protocols
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Calibration
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Chromatography, High Pressure Liquid
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Deoxycytidine / administration & dosage
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Deoxycytidine / analogs & derivatives
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Deoxycytidine / blood
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Docetaxel
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Gemcitabine
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Humans
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Methotrexate / administration & dosage
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Methotrexate / blood
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Paclitaxel / analogs & derivatives*
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Paclitaxel / blood
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Reproducibility of Results
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Solvents
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Spectrophotometry, Ultraviolet
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Taxoids*
Substances
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Antimetabolites, Antineoplastic
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Antineoplastic Agents, Phytogenic
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Solvents
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Taxoids
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Deoxycytidine
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Docetaxel
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Paclitaxel
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Methotrexate
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Gemcitabine