A nude mouse model of human osteosarcoma lung metastases for evaluating new therapeutic strategies

Clin Exp Metastasis. 1999;17(6):501-6. doi: 10.1023/a:1006623001465.


The purpose of these studies was to develop a metastatic osteosarcoma nude mouse model to evaluate the in vivo efficacy of new therapeutic compounds. Human SAOS-2 osteosarcoma cells (10(6) cells) were injected i.v. into nude mice. Cells isolated from a rare pulmonary metastases 6 months later were established (SAOS-LM1) in culture and re-injected. This procedure was repeated 5 additional times to produce the SAOS-LM6 cell line. Visible pulmonary nodules were present 8 weeks following i.v. injection of 10(6) SAOS-LM6 cells as compared to 17 weeks using SAOS-LM2 cells. Microscopic SAOS-LM6 pulmonary metastases were demonstrated at 6 weeks. Administration of adriamycin on week 9 resulted in regression of macroscopic SAOS-LM6 lung tumors. The ability of the model to be used to evaluate the effectiveness of a biologic agent against microscopic disease was also verified. It was concluded that this model can assess therapeutic efficacy and therefore, may have a role in investigating the potential of novel approaches aimed at eliminating pulmonary metastatic osteosarcoma.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antineoplastic Agents / therapeutic use
  • Cytokines / therapeutic use
  • Disease Models, Animal
  • Doxorubicin / therapeutic use
  • Drug Screening Assays, Antitumor
  • Etoposide / therapeutic use
  • Humans
  • Lung Neoplasms / drug therapy*
  • Lung Neoplasms / secondary
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Osteosarcoma / pathology*
  • Vinblastine / therapeutic use


  • Antineoplastic Agents
  • Cytokines
  • Vinblastine
  • Etoposide
  • Doxorubicin