In Vitro and in Vivo Inhibition of Liver Cancer Cells by 1,25-dihydroxyvitamin D3

Cancer Lett. 2000 Apr 3;151(1):97-102. doi: 10.1016/s0304-3835(99)00416-4.

Abstract

Inhibitory effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on the proliferation of a variety of cancer cell lines have been extensively reported. We have studied the effect of 1,25-(OH)2D3 (10(-11)-10(-6) M) on the proliferation of a number of human and rat liver cancer cell lines. Additionally, the effect of 1,25-(OH)2D3 (0.02-0.5 microg/kg per day) on the rate of growth of liver cancer cell line xenografts in nude mice was also investigated. In vitro, proliferation of Hep-3B, PLC/PRF/5, and SKHEP-1 cells was significantly inhibited by 1,25-(OH)2D3, while HTC and Novikoff cells were more resistant to the inhibitory effects of the drug. In vivo, treatment of SKHEP-1 tumor bearing nude mice with different doses of 1,25-(OH)2D3 significantly retarded tumor growth without the development of hypercalcemia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / therapeutic use
  • Antineoplastic Agents / toxicity
  • Calcitriol / therapeutic use*
  • Calcitriol / toxicity*
  • Carcinoma, Hepatocellular / drug therapy
  • Carcinoma, Hepatocellular / pathology*
  • Cell Division / drug effects
  • DNA, Neoplasm / biosynthesis
  • Humans
  • Liver Neoplasms / drug therapy
  • Liver Neoplasms / pathology*
  • Mice
  • Mice, Nude
  • Rats
  • Thymidine / metabolism
  • Transplantation, Heterologous
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • DNA, Neoplasm
  • Calcitriol
  • Thymidine