Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

J Clin Invest. 2000 Apr;105(8):1085-93. doi: 10.1172/JCI8641.


Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the platelike trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. In addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone Marrow Cells / cytology
  • Bone and Bones / physiology*
  • Cells, Cultured
  • Femur
  • Fibroblast Growth Factor 2 / genetics
  • Fibroblast Growth Factor 2 / physiology*
  • Mice
  • Mice, Knockout
  • Osteoblasts / cytology
  • Osteogenesis / physiology
  • RNA, Messenger
  • Skull / cytology
  • Tibia


  • RNA, Messenger
  • Fibroblast Growth Factor 2
  • Alkaline Phosphatase