Estrogen receptor-mediated effects of a xenoestrogen, bisphenol A, on preimplantation mouse embryos

Biochem Biophys Res Commun. 2000 Apr 21;270(3):918-21. doi: 10.1006/bbrc.2000.2548.


The effects of bisphenol A, a xenoestrogen widely used in industry and dentistry, were studied in early preimplantation mouse embryos. Two-cell mouse embryos were cultured with 100 pM to 100 microM bisphenol A with or without 100 nM tamoxifen and evaluated at 24-h intervals for their development to eight-cell and blastocyst stages. At 72 h, blastocysts were cultured for another 48 h without bisphenol A, and surface areas of trophoblast spread were measured. At 24 h, more embryos exposed to 3 nM bisphenol A than to controls had reached the eight-cell stage. At 48 h, more embryos exposed to 1 nM and 3 nM bisphenol A than to controls had become blastocysts. At 100 microM, bisphenol A decreased frequency of development to blastocysts. Tamoxifen counteracted both stimulatory and inhibitory effects of bisphenol A on blastocyst formation. Although bisphenol A did not alter blastocyst morphology or cell number, early exposure to 100 microM bisphenol A increased subsequent trophoblast areas. These findings suggest that bisphenol A may not only effect early embryonic development via estrogen receptors even at low, environmentally relevant doses, but also exert some late effects on subsequent development of these embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzhydryl Compounds
  • Blastocyst / cytology
  • Blastocyst / drug effects*
  • Blastocyst / physiology
  • Cell Division / drug effects
  • Embryonic and Fetal Development / drug effects*
  • Estrogens, Non-Steroidal / pharmacology*
  • Female
  • Male
  • Mice
  • Mice, Inbred Strains
  • Phenols / pharmacology*
  • Pregnancy
  • Receptors, Estrogen / drug effects
  • Receptors, Estrogen / physiology*
  • Tamoxifen / pharmacology*
  • Trophoblasts / drug effects
  • Trophoblasts / physiology


  • Benzhydryl Compounds
  • Estrogens, Non-Steroidal
  • Phenols
  • Receptors, Estrogen
  • Tamoxifen
  • bisphenol A