Phosphorylation of the 105-kDa heat shock proteins, HSP105alpha and HSP105beta, by casein kinase II

Biochem Biophys Res Commun. 2000 Apr 21;270(3):927-31. doi: 10.1006/bbrc.2000.2541.


The 105-kDa heat shock protein alpha (HSP105alpha) and HSP105beta are mammalian heat shock proteins that belong to the HSP105/HSP110 family. Both HSP105alpha and HSP105beta consist of acidic and basic isoforms. Here we report that the acidic isoforms are serine phosphorylated HSP105alpha or HSP105beta. Furthermore, using an in-gel kinase assay with HSP105alpha or HSP105beta as the substrate, the protein kinase that phosphorylates HSP105alpha and HSP105beta was identified as casein kinase II. Since phosphorylated HSP105alpha is especially prominent in the brain compared to other tissues of mice and rats, the phosphorylation of HSP105alpha by casein kinase II may be biologically significant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Casein Kinase II
  • Cell Line
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • HSP110 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins / metabolism*
  • Male
  • Mice
  • Organ Specificity
  • Phosphoric Acids / metabolism
  • Phosphorylation
  • Phosphoserine
  • Protein Isoforms / metabolism
  • Protein Kinases / metabolism
  • Protein-Serine-Threonine Kinases / metabolism*
  • Rats
  • Recombinant Proteins / metabolism
  • Substrate Specificity
  • Testis / metabolism


  • HSP110 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins
  • Hsp105 protein, mouse
  • Phosphoric Acids
  • Protein Isoforms
  • Recombinant Proteins
  • Phosphoserine
  • phosphoric acid
  • Protein Kinases
  • Casein Kinase II
  • Protein-Serine-Threonine Kinases
  • Cyclic AMP-Dependent Protein Kinases