Radiosynthesis of 3'-deoxy-3'-[(18)F]fluorothymidine: [(18)F]FLT for imaging of cellular proliferation in vivo

Nucl Med Biol. 2000 Feb;27(2):143-56. doi: 10.1016/s0969-8051(99)00104-3.


A reliable radiosynthesis of 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT) has been developed based on [(18)10 mCi (370 MBq) of radiochemically pure [(18)1 Ci/micromol (37 GBq/micromol) at EOS within 100 min and in 13% radiochemical yield (end of bombardment (EOB); 7% end of synthesis (EOS)). [(18)F]FLT has been designed as a new positron emission tomography imaging agent for visualizing cellular proliferation in vivo based on the metabolism of thymidine.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Division
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Dideoxynucleosides / chemical synthesis*
  • Fluorine Radioisotopes
  • Indicators and Reagents
  • Isotope Labeling / methods
  • Molecular Conformation
  • Radiopharmaceuticals / chemical synthesis*
  • Spectrophotometry, Ultraviolet
  • Thymidine / metabolism
  • X-Ray Diffraction


  • Dideoxynucleosides
  • Fluorine Radioisotopes
  • Indicators and Reagents
  • Radiopharmaceuticals
  • alovudine
  • Thymidine