Lysosomotropic agents and cysteine protease inhibitors inhibit scrapie-associated prion protein accumulation

K Doh-Ura; T Iwaki; B Caughey

doi:10.1128/jvi.74.10.4894-4897.2000

Lysosomotropic agents and cysteine protease inhibitors inhibit scrapie-associated prion protein accumulation

J Virol. 2000 May;74(10):4894-7. doi: 10.1128/jvi.74.10.4894-4897.2000.

Authors

K Doh-Ura¹, T Iwaki, B Caughey

Affiliation

¹ Department of Neuropathology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. doh-ura@np.med.kyushu-u.ac.jp

Abstract

We report that lysosomotropic agents and cysteine protease inhibitors inhibited protease-resistant prion protein accumulation in scrapie-infected neuroblastoma cells. The inhibition occurred without either apparent effects on normal prion protein biosynthesis or turnover or direct interactions with prion protein molecules. The findings introduce two new classes of inhibitors of the formation of protease-resistant prion protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cysteine Proteinase Inhibitors / pharmacology*
Inhibitory Concentration 50
Leucine / analogs & derivatives*
Leucine / pharmacology
Lysosomes / drug effects
Lysosomes / metabolism
Neuroblastoma
PrPSc Proteins / drug effects*
PrPSc Proteins / metabolism*
Quinacrine / pharmacology*
Tumor Cells, Cultured

Substances

Cysteine Proteinase Inhibitors
PrPSc Proteins
Leucine
Quinacrine
aloxistatin