Different influence of macrophage migration inhibitory factor (MIF) in signal transduction pathway of various T cell subsets

Immunobiology. 2000 Jan;201(3-4):356-67. doi: 10.1016/s0171-2985(00)80090-x.


It has been shown that macrophage migration inhibitory factor (MIF) modulates not only macrophage functions, but also T cell functions. However, detailed analysis of the MIF function on responses of various T cell subpopulations remained to be elucidated. In this report, using a neutralizing anti-MIF monoclonal antibody (mAb) we examined MIF functions on various T cell lineages. It was shown that anti-MIF mAb inhibited antigen-specific responses of both IFN-gamma producing and IL-4 producing T cells. The inhibition appeared to be related to blockade of the signal pathway via T cell receptor (TCR) but not that via IL-2 receptor (IL-2R). However, the anti-MIF mAb showed no inhibitory effect on NK-T cell responses stimulated through TCR. These results suggest that MIF is involved in the signal pathway via TCR in mainstream T cells but not in NK-T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity / immunology
  • Antigens / immunology
  • Antigens, Surface
  • Cell Division
  • Cell Line
  • Humans
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / metabolism
  • Lectins, C-Type
  • Lymphocyte Activation
  • Macrophage Migration-Inhibitory Factors / biosynthesis
  • Macrophage Migration-Inhibitory Factors / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • NK Cell Lectin-Like Receptor Subfamily B
  • Proteins / immunology
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Interleukin-2 / immunology
  • Signal Transduction / immunology*
  • T-Lymphocyte Subsets / immunology*


  • Antibodies, Monoclonal
  • Antigens
  • Antigens, Surface
  • KLRB1 protein, human
  • Lectins, C-Type
  • Macrophage Migration-Inhibitory Factors
  • NK Cell Lectin-Like Receptor Subfamily B
  • Proteins
  • Receptors, Antigen, T-Cell
  • Receptors, Interleukin-2