Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

Biochem Biophys Res Commun. 2000 Apr 29;271(1):164-9. doi: 10.1006/bbrc.2000.2553.

Abstract

One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / metabolism
  • Fluorescent Antibody Technique, Indirect
  • HeLa Cells
  • Heat-Shock Proteins / metabolism*
  • Humans
  • Microscopy, Confocal
  • Microtubules / metabolism*
  • Mitosis
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Binding
  • Sequence Homology, Amino Acid
  • Tubulin / metabolism*

Substances

  • Antibodies, Monoclonal
  • Heat-Shock Proteins
  • Tubulin