Transposon targeting determined by resolvase

FEMS Microbiol Lett. 2000 May 1;186(1):55-9. doi: 10.1111/j.1574-6968.2000.tb09081.x.


The Mu-related transposon Tn5090, also called Tn402, was observed to be highly selective for targets clustered in or close to recombination sites of serine-type recombinases in plasmids R388 and RP1. Transposition to the par area of RP1 responded strongly to a deletion in the gene of resolvase ParA. A search in sequence databanks revealed further insertions of Tn5090/Tn402 close to different genes of resolvases. These results imply that the target selection of Tn5090 depends on a property that is shared among several serine recombinases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Conjugation, Genetic
  • DNA Transposable Elements*
  • Escherichia coli / genetics
  • Gene Targeting*
  • Molecular Sequence Data
  • Plasmids / genetics
  • Recombinases
  • Transformation, Genetic
  • Transposases / genetics
  • Transposases / metabolism*
  • Transposon Resolvases


  • DNA Transposable Elements
  • Recombinases
  • Transposases
  • Transposon Resolvases