The thrombogram: monitoring thrombin generation in platelet-rich plasma

Thromb Haemost. 2000 Apr;83(4):589-91.

Abstract

A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a clot or the presence of platelets and direct measurement in platelet rich plasma is possible.

MeSH terms

  • Area Under Curve
  • Blood Coagulation Tests*
  • Coumarins / analysis*
  • Fluorescent Dyes / analysis*
  • Fluorometry / instrumentation
  • Fluorometry / methods*
  • Humans
  • Oligopeptides / analysis*
  • Plasma
  • Platelet Count
  • Thrombin / analysis*

Substances

  • Coumarins
  • Fluorescent Dyes
  • Oligopeptides
  • benzyloxycarbonyl-glycyl--glycyl-arginine-4-methylcoumaryl-7-amide
  • Thrombin