Synthesis of oligonucleotides containing the oxidatively modified kappaB site: unique binding affinity to the NFkappaB p50 homodimer

Nucleic Acids Symp Ser. 1999;(42):33-4. doi: 10.1093/nass/42.1.33.

Abstract

Synthesis of oligonucleotide 26-mers including single 5-formyl-2'-deoxyuridine (1) or 5-formyl-2'-O-methyluridine (2) in place of thymidine at the kappaB site has been accomplished. One of the 26-mers with 1 was critically discriminated by the NFkappaB p50 homodimer in binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • DNA / chemistry
  • Dimerization
  • NF-kappa B / chemistry*
  • NF-kappa B / metabolism*
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligodeoxyribonucleotides / chemistry*
  • Oxidation-Reduction

Substances

  • NF-kappa B
  • Oligodeoxyribonucleotides
  • DNA