Positional effect of single bulge nucleotide on PNA(peptide nucleic acid)/DNA hybrid stability

Nucleic Acids Symp Ser. 1999:(42):95-6. doi: 10.1093/nass/42.1.95.

Abstract

We report positional effect of bulge nucleotide on PNA/DNA hybrid stability. CD spectra showed that PNA/DNA hybrids required at least seven base pairings at a stem region to form a bulged structure. On the other hand, DNA/DNA could form bulged structure when there are only four base pairings adjacent to the bulge nucleotide. We discuss why PNA requests such a many base pairings to form bulged structure from a nearest neighbor standpoint.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Circular Dichroism
  • DNA / chemistry*
  • Drug Stability
  • Nucleic Acid Conformation
  • Nucleic Acid Heteroduplexes / chemistry
  • Oligodeoxyribonucleotides / chemistry*
  • Peptide Nucleic Acids / chemistry*
  • Spectrophotometry, Ultraviolet

Substances

  • Nucleic Acid Heteroduplexes
  • Oligodeoxyribonucleotides
  • Peptide Nucleic Acids
  • DNA