Proteinase-activated receptor-2 (PAR-2): regulation of salivary and pancreatic exocrine secretion in vivo in rats and mice

Br J Pharmacol. 2000 Apr;129(8):1808-14. doi: 10.1038/sj.bjp.0703274.


Proteinase-activated receptor-2 (PAR-2) is expressed throughout the gastrointestinal tract including the pancreas, and may be involved in digestive functions. The aim of our study was to evaluate a potential role for PAR-2 in regulating salivary and pancreatic exocrine secretion in vivo. PAR-2-activating peptides (PAR-2-APs), but not selective PAR-1-APs, administered intravenously, increased salivary secretion in the mouse or rat; this effect of the PAR-2-APs was unaffected by atropine, phentolamine, propranolol or indomethacin. Secretion (amylase) by rat parotid gland slices in vitro was also stimulated by PAR-2-APs and trypsin, but not by activation of other PARs. PAR-2-APs, administered to rats in vivo, caused a prompt effect on pancreatic exocrine secretion. PAR-2 mRNA, known to be present in pancreatic tissue, was also detected in parotid tissue. Our results indicate that in addition to a potential role in regulating cardiovascular and respiratory functions, PAR-2 may also play a general role in vivo for the direct regulation of glandular exocrine secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbachol / pharmacology
  • Cholinergic Agonists
  • Islets of Langerhans / metabolism*
  • Male
  • Mice
  • Oligopeptides / pharmacology
  • Parotid Gland / metabolism
  • Peptides / pharmacology
  • RNA, Messenger / analysis
  • Rats
  • Rats, Wistar
  • Receptor, PAR-2
  • Receptors, Thrombin / genetics
  • Receptors, Thrombin / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Salivary Glands / metabolism*


  • Cholinergic Agonists
  • Oligopeptides
  • Peptides
  • RNA, Messenger
  • Receptor, PAR-2
  • Receptors, Thrombin
  • seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamide
  • Carbachol