The sequences of enterohemorrhagic Escherichia coli and Yersinia pestis that are homologous to the enteroaggregative E. coli heat-stable enterotoxin gene: cross-species transfer in evolution

FEBS Lett. 2000 Apr 21;472(1):22-6. doi: 10.1016/s0014-5793(00)01414-9.


The enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1) gene is widely distributed among diarrheagenic E. coli. In this study, we examined the sequences of enterohemorrhagic E. coli (EHEC) strains by PCR and sequencing. All the EHEC strains possessed the EAST1 gene homologues but with two types of mutations. One of the mutation types was strongly associated with the large outbreak episodes in 1996 in Japan. Sequence comparison showed that the EHEC sequences are a branch of the EAST1 gene sequence family that showed the cross-species transfer in evolution among E. coli and Yersinia pestis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / genetics*
  • Base Sequence
  • DNA, Bacterial / analysis
  • Electrophoresis, Gel, Pulsed-Field
  • Enterotoxins / genetics*
  • Escherichia coli / genetics*
  • Escherichia coli O157 / genetics*
  • Escherichia coli O157 / isolation & purification
  • Escherichia coli Proteins
  • Evolution, Molecular*
  • Humans
  • Molecular Sequence Data
  • Point Mutation
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • Sequence Analysis, DNA
  • Yersinia pestis / genetics*


  • Bacterial Toxins
  • DNA, Bacterial
  • Enterotoxins
  • Escherichia coli Proteins
  • heat stable toxin (E coli)