Identification of the Drosophila melanogaster homologue of the mammalian signal transducer protein, Vav

FEBS Lett. 2000 Apr 21;472(1):99-104. doi: 10.1016/s0014-5793(00)01413-7.

Abstract

Mammalian Vav signal transducer protein couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. We have isolated and characterized the DroVav gene, the homologue of hVav in Drosophila melanogaster. DroVav encodes a protein (793 residues) whose similarity with hVav is 47% and with hVav2 and hVav3 is 45%. DroVav preserves the unique, complex structure of hVav proteins, including the 'calponin homology', dbl homology, pleckstrin homology; SH2 and SH3 domains in addition to regions that are acidic rich, proline rich and cysteine rich. DroVav is located on the X chromosome in polytene interval 18A5;18B and is expressed in all stages of development and in all tissues. In mammalian cells, DroVav is tyrosine-phosphorylated in response to epidermal growth factor receptor (EGFR) induction; in vitro, the DroVav SH2 region is associated with tyrosine-phosphorylated EGFR. Thus, DroVav probably plays a pivotal role as a signal transducer protein during fruit fly development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Blotting, Southern
  • Cell Cycle Proteins*
  • Cell Line
  • Drosophila melanogaster
  • Embryo, Nonmammalian
  • ErbB Receptors / metabolism
  • In Situ Hybridization
  • Larva
  • Mice
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / isolation & purification
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-vav
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Signal Transduction / genetics*
  • X Chromosome

Substances

  • Cell Cycle Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-vav
  • Vav1 protein, mouse
  • ErbB Receptors

Associated data

  • GENBANK/AA736164
  • GENBANK/AF218780