Molecular cloning and functional expression of beta1, 2-xylosyltransferase cDNA from Arabidopsis thaliana

FEBS Lett. 2000 Apr 21;472(1):105-8. doi: 10.1016/s0014-5793(00)01443-5.


The transfer of xylose from UDP-xylose to the core beta-linked mannose of N-linked oligosaccharides by beta1,2-xylosyltransferase (XylT) is a widespread feature of plant glycoproteins which renders them immunogenic and allergenic in man. Here, we report the isolation of the Arabidopsis thaliana XylT gene, which contains two introns and encodes a 60.2 kDa protein with a predicted type II transmembrane protein topology typical for Golgi glycosyltransferases. Upon expression of A. thaliana XylT cDNA in the baculovirus/insect cell system, a recombinant protein was produced that exhibited XylT activity in vitro. Furthermore, the recombinant enzyme displayed XylT activity in vivo in the insect cells, as judged by the acquired cross-reaction of cellular glycoproteins with antibodies against the beta1,2-xylose epitope. The cloned XylT cDNA as well as the recombinant enzyme are essential tools to study the role of beta1,2-xylose in the immunogenicity and allergenicity of plant glycoproteins at the molecular level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arabidopsis / genetics*
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary
  • Molecular Sequence Data
  • Pentosyltransferases / genetics*
  • Pentosyltransferases / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Spodoptera / cytology


  • DNA, Complementary
  • Recombinant Proteins
  • Pentosyltransferases
  • beta 1,2-xylosyltransferase

Associated data

  • GENBANK/AJ272121