Membrane proteins and proteomics: un amour impossible?

Electrophoresis. 2000 Apr;21(6):1054-70. doi: 10.1002/(SICI)1522-2683(20000401)21:6<1054::AID-ELPS1054>3.0.CO;2-8.


Proteome analysis implies the ability to separate proteins as a first step prior to characterization. Thus, the overall performance of the analysis strongly depends on the performance of the separation tool, usually two-dimensional electrophoresis. This review shows how two-dimensional electrophoresis performs with membrane proteins from bacteria or animal or vegetable cells and tissues, the recent progress in this field, and it examines future prospects in this area.

Publication types

  • Review

MeSH terms

  • Animals
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Forecasting
  • Membrane Proteins / analysis*
  • Proteome / analysis*


  • Membrane Proteins
  • Proteome