Recent advancements in surface-enhanced laser desorption/ionization-time of flight-mass spectrometry

Electrophoresis. 2000 Apr;21(6):1164-77. doi: 10.1002/(SICI)1522-2683(20000401)21:6<1164::AID-ELPS1164>3.0.CO;2-0.


The overall history and recent advances in surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) technology is reviewed herein. Fundamentals of SELDI-TOF analysis are presented while drawing comparisons with other laser-based mass spectrometry techniques. The application of SELDI-TOF-MS to functional genomics and biomarker discovery is discussed and exemplified by elucidating a biomarker candidate for prostatic carcinoma. Finally, a short discussion regarding future SELDI requirements and developments is supplied.

Publication types

  • Review

MeSH terms

  • Humans
  • Proteins / analysis*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Proteins