Distribution of rotavirus VP4 genotypes and VP7 serotypes among nonhospitalized and hospitalized patients with gastroenteritis and patients with nosocomially acquired gastroenteritis in Austria

J Clin Microbiol. 2000 May;38(5):1804-6. doi: 10.1128/JCM.38.5.1804-1806.2000.

Abstract

To assess the potential benefits of a reassortant tetravalent rotavirus vaccine, we investigated stool specimens from children in three different groups by reverse transcription-PCR (RT-PCR) for rotavirus G and P types: (i) children not hospitalized with community-acquired rotavirus-acute gastroenteritis (RV-AGE), (ii) children hospitalized for RV-AGE, and (iii) children with nosocomially acquired RV-AGE. From a total of 553 samples investigated, 335 were positive by enzyme-linked immunosorbent assay, of which 294 (88%) were positive by RT-PCR. Among the RT-PCR-positive samples, the predominant types were G1P[8] (84%), followed by G4P[8] (9%) and G3P[8] (2%). No differences between the three groups were observed, suggesting that community vaccination will diminish the most cost-relevant cases of hospitalizations and nosocomial infections.

MeSH terms

  • Austria
  • Capsid / genetics*
  • Capsid Proteins*
  • Child
  • Cross Infection / virology*
  • Gastroenteritis / virology*
  • Genotype
  • Humans
  • Inpatients
  • Outpatients
  • RNA-Binding Proteins / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification*
  • Rotavirus / genetics
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / diagnosis*
  • Serotyping
  • Viral Nonstructural Proteins / genetics*

Substances

  • Capsid Proteins
  • RNA-Binding Proteins
  • VP4 protein, Rotavirus
  • Viral Nonstructural Proteins
  • NS35 protein, rotavirus