Fatty acid membrane composition and activation of glycine-betaine transport in Lactococcus lactis subjected to osmotic stress

Int J Food Microbiol. 2000 Apr 10;55(1-3):47-51. doi: 10.1016/s0168-1605(00)00193-8.


Lactococcus lactis subsp. cremoris NCDO763 accumulates glycine-betaine (betaine) when submitted to an osmotic stress with NaCl. Betaine transport activity increases with the extent of the osmotic upshock but also with growth temperature, and supplementation of the medium by Tween-80. Fatty acid analysis of the lipid fraction of L. lactis NCDO763 reveals significant modifications of the fatty acid composition of the membrane when cells are submitted to osmotic stress, high temperature or Tween-80 medium supplementation. The main modification in L. lactis membrane fatty acid composition in response to high osmolality is the increase of Cyclopropane Fatty Acid (CFA) deltaC19:0, whereas Unsaturated/Saturated ratio remains unchanged.

MeSH terms

  • Betaine / metabolism*
  • Biological Transport
  • Fatty Acids / analysis*
  • Lactococcus lactis / chemistry
  • Lactococcus lactis / metabolism*
  • Membrane Lipids / analysis*
  • Polysorbates / pharmacology
  • Temperature
  • Water-Electrolyte Balance


  • Fatty Acids
  • Membrane Lipids
  • Polysorbates
  • Betaine