The 19-kD antigen and protective immunity in a murine model of tuberculosis

Clin Exp Immunol. 2000 May;120(2):274-9. doi: 10.1046/j.1365-2249.2000.01212.x.


The 19-kD antigen is a cell wall-associated lipoprotein present in Mycobacterium tuberculosis and in bacille Calmette-Guérin (BCG) vaccine strains. Expression of the 19-kD antigen as a recombinant protein in two saprophytic mycobacteria-M. vaccae and M. smegmatis-resulted in abrogation of their ability to confer protection against M. tuberculosis in a murine challenge model, and in their ability to prime a DTH response to cross-reactive mycobacterial antigens. Induction of an immune response to the 19-kD antigen by an alternative approach of DNA vaccination had no effect on subsequent M. tuberculosis challenge. These results are consistent with a model in which the presence of the 19-kD protein has a detrimental effect on the efficacy of vaccination with live mycobacteria. Targeted inactivation of genes encoding selected antigens represents a potential route towards development of improved vaccine candidates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • BCG Vaccine / genetics
  • BCG Vaccine / immunology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Disease Models, Animal
  • Mice
  • Mice, Inbred C57BL
  • Mycobacterium / immunology
  • Mycobacterium smegmatis / immunology
  • Mycobacterium tuberculosis / immunology
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Time Factors
  • Tuberculosis / microbiology
  • Tuberculosis / physiopathology
  • Tuberculosis / prevention & control*
  • Vaccination
  • Vaccines, DNA / genetics
  • Vaccines, DNA / immunology*


  • 19 kDa antigen, Mycobacterium
  • Antigens, Bacterial
  • BCG Vaccine
  • Bacterial Proteins
  • Recombinant Fusion Proteins
  • Vaccines, DNA