Ralstonia solanacearum produces hrp-dependent pili that are required for PopA secretion but not for attachment of bacteria to plant cells

Mol Microbiol. 2000 Apr;36(2):249-60. doi: 10.1046/j.1365-2958.2000.01851.x.


As in many other Gram-negative plant pathogenic bacteria, the Ralstonia solanacearum hrp genes are involved in the production of a type III secretion apparatus that allows the translocation of PopA protein to the external medium. Here, we show that hrp genes are also involved in the biogenesis of pili that are mainly composed of the HrpY protein. These pili are produced at one pole of the bacterium and are also released into the external medium where they can form very long straight bundles. An hrpY mutant is defective in pilus production, impaired in interactions with plants and in secretion of the PopA protein but not in attachment to plant cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Adhesion
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology*
  • Betaproteobacteria / genetics
  • Betaproteobacteria / metabolism
  • Betaproteobacteria / physiology*
  • Fimbriae, Bacterial / chemistry
  • Fimbriae, Bacterial / genetics
  • Fimbriae, Bacterial / metabolism*
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Regulation, Bacterial
  • Microscopy, Electron
  • Microscopy, Interference
  • Molecular Sequence Data
  • Multigene Family
  • Plant Diseases / microbiology*
  • Plants / microbiology*
  • Sequence Analysis, Protein
  • Time Factors


  • Bacterial Proteins