Deoxyribonucleic acid of Cancer pagurus. II. Template activity for a DNA-dependent DNA polymerase of eukaryotic cells

Nucleic Acids Res. 1974 Oct;1(10):1233-47. doi: 10.1093/nar/1.10.1233.


The template activity of Cancer pagurus DNA and its two components (poly d(A-T) and main component) in response to a DNA polymerase purified from regenerating rat liver has been studied and compared to the results previously obtained with synthetic templates. In the double-stranded native state, whole crab DNA and the main component were poor templates. Their replication was increased by thermal denaturation and inhibited by actinomycin. Like the synthetic copolymer poly[d(A-T).d(T-A)], native crab poly d(A-T) could be copied and its duplication was not inhibited by actinomycin. The structural difference between native poly d(A-T) Form I, isolated on a density gradient, and partially renatured poly d(A-T) Form II, isolated on hydroxylapatite, resulted in a modification of their template activity. The kinetic studies of [(3)H] dGMP and [(3)H] dAMP incorporation confirmed the importance of single-stranded regions (particulary dC regions) in the initiation of the in vitro duplication.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Brachyura / chemistry*
  • DNA / isolation & purification*
  • DNA / metabolism
  • DNA Replication* / drug effects
  • DNA, Single-Stranded / metabolism
  • DNA-Directed DNA Polymerase / metabolism*
  • Dactinomycin / pharmacology
  • Endodeoxyribonucleases / metabolism
  • Eukaryotic Cells / metabolism
  • Fungal Proteins / metabolism
  • Hot Temperature
  • Kinetics
  • Liver / enzymology
  • Liver Regeneration
  • Neurospora crassa / enzymology
  • Nucleic Acid Denaturation
  • Nucleic Acid Synthesis Inhibitors / pharmacology
  • Poly dA-dT / chemical synthesis
  • Poly dA-dT / isolation & purification
  • Poly dA-dT / metabolism*
  • Rats
  • Templates, Genetic


  • DNA, Single-Stranded
  • Fungal Proteins
  • Nucleic Acid Synthesis Inhibitors
  • Dactinomycin
  • Poly dA-dT
  • DNA
  • DNA-Directed DNA Polymerase
  • Endodeoxyribonucleases