The effect of bisulfite modification on the template activity of DNA for DNA polymerase I

Nucleic Acids Res. 1974 Jul;1(7):889-99. doi: 10.1093/nar/1.7.889.

Abstract

Cytosine residues of poly(C) and heat-denatured calf thymus DNA were transformed into 5,6-dihydrouracil-6-sulfonate (U(SO(-) (3))) residues by treatment with bisulfite. The poly(U(SO(-) (3))(2), C(3)) and poly(U(SO(-) (3))(9), C(1)) prepared did not form inter-base binding with either poly(A) or poly(I) as judged by the absence of hypochromicity in ultraviolet absorbance. U(SO(-) (3)) residues in the DNA inactivated it to serve as template for E.coli DNA polymerase I, while the template activity was restored by conversion of the U(SO(-) (3)) residues into U.

MeSH terms

  • Animals
  • Cattle
  • DNA / chemistry
  • DNA / drug effects*
  • DNA / metabolism*
  • DNA Polymerase I / metabolism*
  • Escherichia coli / enzymology
  • In Vitro Techniques
  • Mutagenesis
  • Poly A / metabolism
  • Poly C / chemistry
  • Poly C / metabolism
  • Poly I / metabolism
  • Poly U / chemistry
  • Poly U / metabolism
  • Sulfites / toxicity*

Substances

  • Sulfites
  • Poly A
  • Poly I
  • poly(U-C)
  • Poly U
  • Poly C
  • DNA
  • DNA Polymerase I