Smart and biofunctional streptavidin

Biomol Eng. 1999 Dec 31;16(1-4):93-9. doi: 10.1016/s1050-3862(99)00043-1.


The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. While it is extremely useful as the native protein, there are many applications where its function can be improved re-engineering the subunits. We review here our efforts to construct streptavidin tetramers that have 'smart' recognition capabilities, and which display functional peptide sequences. These smart and biofunctional streptavidin derivatives can 'talk' to cells, and 'listen' to external signals which control capture and release of biotinylated molecules.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Affinity Labels
  • Binding Sites
  • Biotechnology
  • Biotin / metabolism
  • Models, Molecular
  • Protein Conformation
  • Protein Engineering
  • Protein Structure, Quaternary
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Streptavidin / chemistry*
  • Streptavidin / genetics
  • Streptavidin / metabolism*


  • Affinity Labels
  • Recombinant Fusion Proteins
  • Biotin
  • Streptavidin