Neuronal differentiation of cryopreserved neural progenitor cells derived from mouse embryonic stem cells

Biochem Biophys Res Commun. 2000 May 10;271(2):418-21. doi: 10.1006/bbrc.2000.2631.


Embryonic stem cells (ES cells) are developmentally pluripotent cells isolated from pre-implantation mammalian embryos. In cell culture ES cells can be easily differentiated to generate cultures of neural progenitors. We present a simple method for the cryopreservation of these ES-derived neural progenitors. Cryopreserved neural progenitor stocks can be thawed, expanded with FGF2, and differentiated into functional neurons. This method will facilitate studies using ES-derived neural progenitor cells as a cell culture model system for neural development and differentiation. It will also aid studies designed to test the ability of these progenitor cells to functionally engraft and repair damaged neural tissue.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cells, Cultured
  • Cryopreservation*
  • Electrophysiology
  • Embryo, Mammalian / cytology*
  • Fibroblast Growth Factor 2 / metabolism
  • Fibroblasts / metabolism
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Mice
  • Neurons / cytology*
  • Phenotype
  • Stem Cells / cytology*
  • Stem Cells / metabolism*


  • Fibroblast Growth Factor 2